Team:Slovenia/SafetyMechanismsMicrocapsuleDegradation ...- liquid detergent and alginate lyase ,Alginate lyase can break down the alginate polymer of microcapsules, ideally leaving no trace of our biopharmaceutical delivery system. Alginate lyases are not present in the mammalian genome but have been found in bacteria, algae and marine mollusks. Alginate lyase is able to depolymerize the alginate crosslinked by calcium (Breguet et al., 2007).Pseudomonas Infection Responsive Liquid Crystals for ...The anti-biofilm activity of alginate lyase and gentamicin from the liquid crystals was sustained for 2 days and equivalent to the respective unformulated solution treatments. Accordingly, GMO based liquid crystals are a promising responsive delivery system for alginate lyase and gentamicin to combat topical Pseudomonas infections.



Ingrédients détergents liquides

Persil® Laundry Detergent | Persil® ProClean®- Ingrédients détergents liquides ,Persil® ProClean® laundry detergents are packed with Pro-Power technology to help fight tough laundry stains.Laundry Detergent Ingredients and How They WorkLaundry detergents have come a long way since the first bar soaps made from animal fat and lye were offered for sale in the 1700s.

Contact the supplier

(PDF) Alginate Lyases from Alginate-Degrading Vibrio ...

AlyA enzyme and each of the alginate lyase domains independently overex- pressed was evaluated in 0.05% of alginate dissolved in 20 mM APT, pH 8.5, with 250 mM NaCl and incubated for 20 min at 20°C.

Contact the supplier

Isolation and characterization of alginate-degrading ...

an alginate lyase that acts on the 4-O-linked glycosidic linkage of alginate (Iwamoto et al. 2001). Alginate lyases ... ria in the agar plate were further checked in a liquid media containing the same components described above except that the alginate content was 5 g l)1.

Contact the supplier

Purification and Characterization of a Novel Alginate ...

These characteristics suggested that AlgA is an endolytic polyM-specific alginate lyase (EC 4.2.2.3). The products of alginate and polyM degradation by AlgA were purified and identified through fast protein liquid chromatography and electrospray ionization mass spectrometry, which revealed that AlgA mainly produced disaccharides, trisaccharides ...

Contact the supplier

Raw materials used to produce liquid detergents

Raw materials used to produce liquid detergents ,How to Make Liquid Soap. Making liquid soap is more complicated than other cleaning product, but the result will be worth. The steps for making liquid shop are : Mixing the Lye-Water Solution and the Oils for the Liquid Soap, after heating the oil up approximately to 160 degrees celcius mix the ...

Contact the supplier

Sparrho | Interaction of extracellular Pseudomonas lipase ...

(iii) In the presence of alginate the increase in exolipase activity caused by the non-ionic detergent Triton X-100 was drastically reduced. (iv) Exolipase could be rapidly and almost completely harvested from cell-free culture fluid of P. aeruginosa 5940 by ethanolic coprecipitation with alginate.

Contact the supplier

Role of the Pseudomonas fluorescens Alginate Lyase (AlgL ...

Alginate is an industrially widely used polysaccharide produced by brown seaweeds and as an exopolysaccharide by bacteria belonging to the genera Pseudomonas and Azotobacter . The polymer is composed of the two sugar monomers mannuronic acid and guluronic acid (G), and in all these bacteria the genes encoding 12 of the proteins essential for synthesis of the polymer are clustered in the genome.

Contact the supplier

Purification and characterization of a novel alginate ...

alginate lyases (e.g. exo- and endo-types and oligoal-ginate lyase) are generally used to degrade alginate. We herein describe a novel alginate lyase, AlgC-PL7, which belongs to the polysaccharide lyase 7 family. AlgC-PL7 was isolated from the halophilic Gram-negative bacterium Cobetia sp. NAP1 col-lected from the brown algae Padina arborescens ...

Contact the supplier

Structural and Functional Studies on Three Alginate Lyases ...

Keywords:Alginate lyase, chimeric protein, ESI-MS, NMR, site-directed mutagenesis, substrate specificity. Abstract:To date, several microorganisms that can metabolize alginate have been characterized. In these microorganisms, a class of enzymes called alginate lyases (Alys) catalyze the depolymerization of alginate into oligomers via an ...

Contact the supplier

Sigma Aldrich Fine Chemicals Biosciences Alginate Lyase ...

Sigma Aldrich Fine Chemicals Biosciences Alginate Lyase | 9024-15-1 | MFCD03454593 | 100mg ALGINATE LYASE,POWDER, >=10,00

Contact the supplier

ORIGINAL ARTICLE The effect of alginate lyase on the ...

Alginate lyase depolymerized alginate, but calcium and zinc cations at concentrations found in the cystic fibrosis lung reduced enzyme activity. Biofilms formed on agar were partially degraded by alginate lyase, but staining with crystal violet showed that the biomass of biofilms grown in liquid was not significantly affected by the enzyme ...

Contact the supplier

Pseudomonas Infection Responsive Liquid Crystals for ...

The anti-biofilm activity of alginate lyase and gentamicin from the liquid crystals was sustained for 2 days and equivalent to the respective unformulated solution treatments. Accordingly, GMO based liquid crystals are a promising responsive delivery system for alginate lyase and gentamicin to combat topical Pseudomonas infections.

Contact the supplier

Structural and Functional Studies on Three Alginate Lyases ...

Keywords:Alginate lyase, chimeric protein, ESI-MS, NMR, site-directed mutagenesis, substrate specificity. Abstract:To date, several microorganisms that can metabolize alginate have been characterized. In these microorganisms, a class of enzymes called alginate lyases (Alys) catalyze the depolymerization of alginate into oligomers via an ...

Contact the supplier

Lyase from the Marine Bacterium Bacillus

2.2. Purification of Alginate Lyase from Bacillus sp. Alg07 Bacillus sp. Alg07 was cultured in optimized liquid medium for 24 h until its alginate lyase reached the highest activity. The supernatant containing 510 U/mL of alginate lyase was subjected to further purification through the two simple steps of tangential flow filtration ...

Contact the supplier

A rapid, sensitive, simple plate assay for detection of ...

The alginate-lyase-activity area indicated using the Gram's iodine method was found to be larger than that indicated by the CPC method. Both methods (CPC and Gram's iodine) showed the largest alginate lyase activity area for Saccharophagus degradans (ATCC 43961) followed by Microbulbifer mangrovi (KCTC 23483), Bacillus cereus (KF801505) and ...

Contact the supplier

Pseudomonas infection responsive liquid crystals for ...

Treatment with the liquid crystals containing alginate lyase and gentamicin resulted in a greater than 2-log reduction in mucoid Pseudomonas aeruginosa (clinical isolate) biofilm. The anti-biofilm activity of alginate lyase and gentamicin from the liquid crystals was sustained for 2 days and equivalent to the respective unformulated solution ...

Contact the supplier

Sparrho | Interaction of extracellular Pseudomonas lipase ...

(iii) In the presence of alginate the increase in exolipase activity caused by the non-ionic detergent Triton X-100 was drastically reduced. (iv) Exolipase could be rapidly and almost completely harvested from cell-free culture fluid of P. aeruginosa 5940 by ethanolic coprecipitation with alginate.

Contact the supplier

Characterization of a Novel Alginate Lyase from Marine ...

marine drugs Article Characterization of a Novel Alginate Lyase from Marine Bacterium Vibrio furnissii H1 Xiaoyan Zhu 1,2,*, Xiangqian Li 1,2, Hao Shi 1,2, Jia Zhou 1,2, Zhongbiao Tan 1,2, Mengdi Yuan 2, Peng Yao 2 and Xiaoyan Liu 3 1 Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, China; [email protected] ...

Contact the supplier

Lyase from the Marine Bacterium Bacillus

2.2. Purification of Alginate Lyase from Bacillus sp. Alg07 Bacillus sp. Alg07 was cultured in optimized liquid medium for 24 h until its alginate lyase reached the highest activity. The supernatant containing 510 U/mL of alginate lyase was subjected to further purification through the two simple steps of tangential flow filtration ...

Contact the supplier

Detection and Pharmacokinetics of Alginate ...

Preparation of AOs. Sodium alginate (5g) was gradually dissolved in 500ml of distilled water with heating at 50 C, and an aliquot (1ml) of alginate lyase solution (500mg/ml) was added to the alginate solu-tion. The reaction mixture was incubated at 40 C for 3d. During the incubation period, the same volume of fresh enzyme solution was added 3 ...

Contact the supplier